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Catalogue of recombinant enzymes for proteomic research

Class	Product Name	Action Sites	Application	Specification (lyophilized powder)
Sequencing Grade	Recombinant Lysine Endopeptidase (Lys-C)	Specifical cleaving at the carboxyl side of Lysine Residue including Pro-Lys, Asp-Lys and other sequences hard to be identified	①Proteomic research ②Peptide mapping ③Protein sequencing ④Identification of phosphorylation ⑤Analysis of disulfide bond ⑥Analysis of N-glycan	20μg
	Recombinant Lysine Endopeptidase (Lys-C)			20μg，5 vials
	Recombinant Tripsin (TP)	Specifical cleaving at the carboxyl side of Arg and Lys	①Proteomic Research ②Peptide mapping ③Protein sequencing	20μg
	Recombinant Tripsin (TP)	Specifical cleaving at the carboxyl side of Arg and Lys	①Proteomic Research ②Peptide mapping ③Protein sequencing	20μg，5 vials
	RLys-C/RTP mix	Specifical cleaving at the carboxyl side of Arg and Lys	①Peptide mapping, especially suitable for MS analysis with large molecular or high- hydrophobic proteins, with remarkable increase peptides number ②Quality control or analysis of MAb biologicals	20μg
	RLys-C/RTP mix	Specifical cleaving at the carboxyl side of Arg and Lys		20μg，5 vials
	Endoproteinase Glu-C (V8)	Cleaving at the carboxyl side of Glu and Asp. Specific at Glutamyl bond at ammonium acetate（pH 4.0）or ammonium bicarbonate (pH 7.8)	①Proteomic research ②Peptide mapping of insulin analogues ③Peptide mapping of MAb	50μg
	Endoproteinase Glu-C (V8)			50μg，5 vials
	Recombinant Carboxypeptidase B (CPB)	Preferential cleaving C-terminal basic amino acid including Lys, Arg, His	①C-terminal sequencing ②Quality control of Recombinant MAb ③Removing C-terminal His-tag	20μg
	Recombinant Carboxypeptidase B (CPB)			20μg，5 vials
Research and Analysis Use	Recombinant Enterokinase (EK)	Specifical cleaving at the carboxyl side of Asp-Asp-Asp-Asp-Lys	①Removing fusion tag of recombinant protein ②Activating the enzymatic digestion of trypsinogen	50μg
	Recombinant Enterokinase (EK)			50μg, 5 vials
	Recombinant Tobaccoetchvirus protease (TEV)	Specifical cleaving at the carboxyl side of Glu-Asn-Leu-Tyr-Phe-Gln-Gly	Removing fusion tag of recombinant protein	50μg
	Recombinant Tobaccoetchvirus protease (TEV)		Removing fusion tag of recombinant protein	50μg，5 vials

Catalogue of recombinant enzymes for industrial manufacture

Product	Character	Action Sites	Application	Specification (lyophilized powder)	Activity
Recombinant Lysine Endopeptidase (Lys-C)	Gene Source: L. enzymogenes Expression host: E.coli Tag-free	Cleaving at the carboxyl side of lysine residues	①Enzymatic proinsulin digestion and transpeptidation of recombinant insulin or its analogues（e.g. Insulin aspart） ②Specific enzymatic digestion of recombinant polypeptide drugs	10mg	≥3AU/mg
Recombinant Lysine Endopeptidase (Lys-C)		Cleaving at the carboxyl side of lysine residues		1g	≥3AU/mg
Recombinant Tripsin (TP)	Gene Source: Swine Expression host: P. pichia Tag-free	Cleaving at the carboxyl side of lysine or arginine residues	① Enzymatic proinsulin digestion and transpeptidation of recombinant insulin ②Digestive treatment Padhereut cell cultures ③Tissue enzymatic extraction ④Hydrolysis of peptone preparation	10mg	≥3800USP/mg
Recombinant Tripsin (TP)	Gene Source: Swine Expression host: P. pichia Tag-free			1g	≥3800USP/mg
Recombinant Kexin (Kex2)	Gene Source: Yeast Expression host: P. pichia Tag-free	Cleaving at the carboxyl side of Arg-Arg or Lys-Arg	① Specific enzymatic processing preparation of proinsulin glargine ② Enzymatic preparation of recombinant peptide by fusion expression	10mg	≥10U/mg
Recombinant Kexin (Kex2)	Gene Source: Yeast Expression host: P. pichia Tag-free	Cleaving at the carboxyl side of Arg-Arg or Lys-Arg		1g	≥10U/mg
Carboxypeptidase B (CPB)	Gene Source: mouse pancreas Expression host: P. pichia Tag-free	Removes C-terminal Lys, Arg, His	① Removing C-terminal basic amino acid of protein drug (e.g. recombinant insulin or analogues) or others	10mg	≥150U/mg
Carboxypeptidase B (CPB)		Removes C-terminal Lys, Arg, His		1g	≥150U/mg
Recombinant Enterokinase (EK)	Gene Source: Swine Expression host: P. pichia Tag-free	Highly recognizing sequence ‘DDDDK’ and cleaving at the lysine (K)	Used as a processing enzyme in the fusion expressing polypeptide	10mg	≥10000U/mg
Recombinant Enterokinase (EK)	Gene Source: Swine Expression host: P. pichia Tag-free			1g	≥10000U/mg
Recombinant Tobaccoetchvirus protease (TEV)	Expression host: E. coli With His-tag	Recognizing the carboxyl side of sequence Glu-Asn-Leu- Tyr-Phe-Gln-(Gly/Ser) and cleaving between the Gln and Gly/Ser residues.	It can be used as a processing enzyme in the fusion expression of polypeptide drugs or others	10mg	≥1000U/mg
Recombinant Tobaccoetchvirus protease (TEV)	Expression host: E. coli With His-tag			1g	≥1000U/mg
Recombinant Methionine Adenosyltransferase (MAT)	Expression host: E. coli With His-tag	catalyzing the formation of S-adenosyl methionine by methionine and ATP	It can be used to produce S-adenosyl methionine	10mg	≥0.6U/mg
Recombinant Methionine Adenosyltransferase (MAT)	Expression host: E. coli With His-tag		It can be used to produce S-adenosyl methionine	1g	≥0.6U/mg
Recombinant Arginase	Gene Source: Homo sapiens Expression host: E. coli Tag-free	Converting L-arginine into L-ornithine and urea	Used to produce natural ornithine	10mg	≥20U/mg
Recombinant Arginase	Gene Source: Homo sapiens Expression host: E. coli Tag-free	Converting L-arginine into L-ornithine and urea	Used to produce natural ornithine	1g	≥20U/mg
Recombinant Arginine Deiminoenzyme	Gene Source: Mycoplasma hominis Expression host: E. coli Tag-free	Catalyzing the hydrolysis of L-arginine to produce citrulline	Used to produce natural citrulline	10mg	≥30U/mg
Recombinant Arginine Deiminoenzyme			Used to produce natural citrulline	1g	≥30U/mg
Recombinant 4-Hydroxyl Prolinase (P4H)	Gene Source: ascomycetes Expression host: E. coli Tag-free	Catalyzing L-proline into L-hydroxyproline	①Used to produce L-hydroxyproline； ②Protine Hydroxylation of collagen or analogues	10mg	≥1.0U/mg
Recombinant 4-Hydroxyl Prolinase (P4H)	Gene Source: ascomycetes Expression host: E. coli Tag-free	Catalyzing L-proline into L-hydroxyproline		1g	≥1.0U/mg
Recombinant Human Hyaluronidase (PH20)	Gene Source: Homo sapiens Expression host: CHO Tag-free	Catalyzing the hydrolysis of large molecule hyaluronic acid	Produce low-molecular active hyaluronic acid (for cosmetics)	0.1mg	≥50, 000U/mg
Recombinant Human Hyaluronidase (PH20)	Gene Source: Homo sapiens Expression host: CHO Tag-free	Catalyzing the hydrolysis of large molecule hyaluronic acid		0.5mg	≥50, 000U/mg

We can provide customized package according to customers requirement

——Catalogue of recombinant enzymes for diagnosis——

Product Name	Appearance	Source	Substrate-product	Application	Activity
Urease	White lyophilized powder	Pseudomonas	Urea—NH3	Enzymatic determination of urea in clinical diagnosis	≥100U/mg
Sarcosine Oxidase	Yellow lyophilized powder	Bacillus sp. BSD-8	Sarcosine—Glycine&Formaldehyde	Enzymatic determination of creatinine, creatine, and sarcosine when coupled with creatinine amidohydrolase and creatine amidinohydrolase	≥8.0U/mg
Creatine Amidinohydrolase	White lyophilized powder	Pseudomonas putida	Creatine—Sarcosine	Enzymatic determination of creatinine when couple with creatine amidinohydrolase in clinical diagnosis	≥40U/mg
Creatinine Amidohydrolase	White lyophilized powder	Pseudomonas	Creatinine—Creatine	Enzymatic determination of creatinine when coupled with creatine amidinohydrolase and sarcosine oxidase in clinical analysis	≥150U/mg
Glycerol Dehydrogenase	White lyophilized powder	Escherichia coli CFT073	Glycerol—Dihydroxyacetone

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